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Although the derivation of closed-form optimal expressions is challenging, the optimal value can be obtained by using numerical simulations which are presented in the next section.
In their work, a special interest is given to the performance analysis of the NLMS algorithm (e.g., see Chapters 7 and 13 from [1]), in terms of developing optimal expressions for its control parameters, i.e., the normalized step-size and regularization term.
The optimal expressions for each protein were in a hierarchical order: namely Flag-Rta (24 48 h), EBV immediate-early protein BZLF1 (48 72 h), early proteins BMRF1 and BHRF1 (72–144 h), and late membrane protein gp350/220 (120–144 h).
To investigate the osteoinduced capability via the cotransfection of BMP-2 and VEGF, the C2C12 cell line was chosen for further in vitro coculturing with BMSCs due to its optimal expressions of both BMP-2 and VEGF.
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Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography.
Consider a gene expressed at this optimal expression level, ϵopt.
A3B was also necessary for optimal expression of ER-regulated genes in the ER-expressing MDA-MB-231 lines.
We increased the amount of plasmid DNA to improve transfection efficiency, and we optimized the TCR/CD3 plasmid DNA ratio to ensure optimal expression of both TCR and CD3.
Here health represents a full and optimal expression of the individual's capacities and uniqueness, while illness represents a restricted or limited expression of the self and occurs in response to an interaction between internal and environmental conditions.
To obtain the optimal expression element, successive investigation of RBS sequences and promoters was conducted.
Furthermore, expression profiling can facilitate the design of optimal expression plasmids for use in specific cancers.
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