Sentence examples for optimal expression was from inspiring English sources

Based on the analysis of cell lysate from the small scale expression of five antibodies, we have determined that best expression levels can be obtained using only two sets of conditions: for PA38, PA64 and 4G2, optimal expression was observed from the high copy plasmid, induced at OD600 1.0 with 20 ng/ml inducer.

The optimal expression was 21.08 mg/L at 12 h cultivation.

In this experiment, the injured rat endometrial cells were treated with various concentrations of quercetin, the results showed that the expression of CYP2B1 increased gradually with the gradient quercetin treatment, and the optimal expression was 50  μmol/L quercetin treated for 24 h.

Conditions required for optimal expression were determined to be between 200 300 V/cm and 150 ms. An additional consideration for establishing a MEA delivered DNA vaccine is choosing the appropriate animal model.

Although the derivation of closed-form optimal expressions is challenging, the optimal value can be obtained by using numerical simulations which are presented in the next section.

The ease with which close-to-optimal expression is obtainable in a single step with the new approach, coupled with the target protein-independent selection of expressing cells, offsets some of the risk in producing hard-to-assay proteins, as exemplified by our expression of biotinylatable CCL18.

The optimal Sh expression was then used to predict the behavior of systems with a larger channel size or longer bed; the model predictions showed good agreement with experimental results from real WMH reactors.

Optimal protein expression was obtained by reduction of the fermentation temperature and addition of casamino acids as a supplemental nitrogen source and to minimize the activity of yeast produced proteases.

Optimal Fos expression was observed when the animal was subjected to linear acceleration at 1.5 Hz for 90 min.

Taken together, these data suggested that optimal rGRA5 expression was achieved following induction with 1.0 mM IPTG for 2 hours.

Optimal protein expression was obtained by culturing E. coli TOP10 cells harboring pBADHAS in TB supplemented with 50 μg mL−1 ampicillin and 0.02% (w/v) l-arabinose at 37 °C for 12 h.