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Exact(25)
Consider a gene expressed at this optimal expression level, ϵopt.
Although the RGD peptide remains equally solvent-exposed and immunoreactive at different DNA protein ratios, an optimal expression level of cell-delivered genes and integrin-binding specificity are both achieved at 0.02 μg of DNA per μg of protein, indicative of influences of the packaged nucleic acid on the interaction between filled vehicles and the receptors of target cells.
Since nearly all constructs are correct, the library can be screened directly for optimal expression level for this particular gene, or be used for the next level of cloning in which several genes or again gene libraries are assembled.
Unlike the embryos (see Fig. 1B), MEFs in cell culture adjusted their vinculin expression, and it appears that for cellular invasiveness there is an optimal expression level of vinculin protein.
The population grown without IPTG reached its optimal expression level in ~200 generations.
We assume that, in each given environment, there is an optimal expression level μ*.
Similar(35)
SPLs inhibit tillering in general, but promote panicle branching at optimal expression levels to increase grain number.
With the current advancements in DNA technology, from cheap synthesis to synthetic biology, it is now possible to design, in a modular and combinatorial fashion, not only the genes that will structure the heterologous metabolic pathways, but also the regulatory elements that will ensure the optimal expression levels of such genes.
Some genes require optimal expression levels to display their functional properties [25], [26], [27], and GABAB1m and n may also need optimal expression ranges to inhibit function.
Genes that have differential growth effects are likely to have different optimal expression levels in different environments, because the experimental protocol manipulates expression level in an environment independent way.
This process shapes the codon usage patterns for selected genes to gain optimal expression levels in response to changing environments.
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