Sentence examples for optimal expression in from inspiring English sources
Alternatively the HA gene was codon-optimized for optimal expression in mammalian cells by the UpGene codon optimization algorithm (http://www.vectorcore.pitt.edu/upgene.html) and synthesized by GenScript.
First, we codon-harmonized the gene sequence of PfCelTOS for optimal expression in E. coli based on our previously published algorithm [9].
One possible explanation is that some regulatory elements necessary for optimal expression in pollen were missing in the chosen promoter region.
For optimal expression in Lactococcus lactis [27], residues 2 14 and 309 315 were removed by PCR, including the His-tag, and the resulting genes were cloned into the expression vector pNZ8048 under the control of a nisin A-inducible promoter [28].
Genes that have differential growth effects are likely to have different optimal expression levels in different environments, because the experimental protocol manipulates expression level in an environment independent way.
The population grown without IPTG reached its optimal expression level in ~200 generations.
This process shapes the codon usage patterns for selected genes to gain optimal expression levels in response to changing environments.
The balanced and optimal expression of genes in an engineered pathway is a prerequisite for the overproduction of a natural compound in a customized strain.
To obtain an optimal expression of IncA in human cells full-length DNA sequence of IncA of Cp. psittaci was optimized for human codon usage (sequence available on demand), in vitro synthesized (GenScript) and cloned as an EcoRI-NotI-fragment into pcDNA3.1/myc-His A (Invitrogen).
Noting differences between in vitro and in vivo data, in general, optimal expression patterns concerning chondrogenic differentiation were achieved by few passages, medium cellular yield, short membrane-holding time.
Furthermore, expression profiling can facilitate the design of optimal expression plasmids for use in specific cancers.
