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Alternatively the HA gene was codon-optimized for optimal expression in mammalian cells by the UpGene codon optimization algorithm (http://www.vectorcore.pitt.edu/upgene.html) and synthesized by GenScript.
Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography.
Consider a gene expressed at this optimal expression level, ϵopt.
A3B was also necessary for optimal expression of ER-regulated genes in the ER-expressing MDA-MB-231 lines.
Desai, P. N., Shrivastava, N. & Padh, H. Production of heterologous proteins in plants: Strategies for optimal expression.
These data will facilitate optimal expression cassette design for salivary gland gene transfer.
Optimal expression conditions were identified enabling generation of milligram-scale quantities of active RNase U2.
Furthermore, expression profiling can facilitate the design of optimal expression plasmids for use in specific cancers.
Arêde, P., Milheiriço, C., de Lencastre, H. & Oliveira, D. C. The anti-repressor MecR2 promotes the proteolysis of the mecA repressor and enables optimal expression of β-lactam resistance in MRSA.
The relationship between the mazF transcription rate αf and the FP translation rate (ktransFP=ktrans[rFP]) is non-monotonic (Supplementary Fig. 13a), indicating that there is an optimal expression level of MazF to maximize resource redistribution activity.
Three expressions for the Sherwood number that are typically used for honeycomb, wire-mesh gauze and packed bed reactors were examined to determine the optimal expression for WMHs.
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