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Optimal equilibration time and equilibration temperature are necessary to complete reactions and to achieve easy phase separation and preconcentration as efficiently as possible.
Optimal equilibration of the standard was found with 1 h incubation, and this was used for the remaining data presented in this paper.
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Based on these results, we numerically predict and compare optimal multistep equilibration protocols that minimize the cell-based cumulative toxicity cost function and the damage due to excessive osmotic gradients at the tissue boundary.
As determined in preliminary experiments, tissues were set at optimal length by equilibration against a passive load of 0.8 g.
Tissues were set at optimal length (Lo) by equilibration against a passive load of 1.5 g in rat aorta, normoxic human and rat pulmonary arteries, and 2.5 g in CH rat pulmonary arteries, as determined for these types of preparation in control experiments (data not shown).
Following a 60 minute equilibration period, an optimal pre-tension of 2.00 g for each chorionic plate arterial ring was utilised [ 4].
The optimal conditions included a 5 min equilibration at 70 °C time in an ultrasonic bath with an extraction time of 60 min.
(498) Both immiscible phases sit one above the other in a temperature-controlled jar and are stirred at an optimal rate that secures sufficiently rapid equilibration in the bulks.
Arterial segments were subjected to a 60 min equilibration period at the predetermined optimal point of its active length-tension curve established at 2 g by measuring the contraction to 30 mM potassium chloride (KCl) at different levels of stretch.
To achieve the optimal positions of the selected residues, a receptor equilibration was subsequently performed allowing for translation, rotation, and torsional variations of receptor's amino acid residues.
After equilibration for 60 min at the optimal resting tension (rat aorta: 1 g; porcine coronary artery: 2 g; bovine coronary artery: 4 g), maximal contractile activity was determined with a depolarizing solution containing 100 mM K+.
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