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Probability of occurrence of "optimal" endurance profile was also determined.
Frequency distribution of the optimal endurance genotype in the male Gorkha soldiers and other Asian and Caucasian populations is shown in Table 8.
With 4% of the male Gorkha soldiers (who are otherwise drawn from the general population) having the optimal endurance profile, this would mean that nearly 1.7 million, out of an estimated 43 million (31 million of Nepalese from Nepal and about 12 million Nepalese domiciled in India, Nepal Census 2011) would have the optimal endurance profile.
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The optimal endurance-associated homozygous genotypic combination of ACE II/ACTN3 XX/CKMM AA/eNOS GG was present in nearly 4% of the male Gorkha soldier population (Additional file 2: Table S2).
They mix sub-optimal endurance with high-octane effort.
Probability of male Gorkha soldiers possessing a theoretically optimal polygenic endurance profile for four candidate polymorphisms was ~3.35% (1 in 30).
Even if new candidate polymorphisms are scored, probability of presence of individuals with optimal genotypes for endurance still remains large in this population.
From a physiological standpoint, the power-duration relationship, characterizing the time for which a given exercise intensity can be sustained, has been proposed to determine the optimal workload in endurance tests [14], [15].
A genotype score (GS) of 2 was assigned to the "optimal" homozygous genotype for endurance.
The probability of any male Gorkha soldier possessing the optimal polygenic profile for endurance at all four loci was ~3.35% with an approximate odds ratio of 1 30 (i.e., one in 30 individuals) (Table 7).
The probability of any lowlander possessing the optimal polygenic profile for endurance at all four loci further reduced to 1.88% with an approximate odds ratio of 1 78 (i.e., one in 78 individuals) (Table 7).
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