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The optimization of the ELISA was done to obtain the optimal dynamic range of the measured antibodies using a single dilution of the serum samples (1 50).
Coinciding with results from previous authors [16, 29] we showed that the system exhibits optimal dynamic range when the branching parameter is equal to one.
These observations and the idea that the brain is a critical system have drawn much attention as critical systems have been shown to exhibit optimal dynamic range and optimal information processing [16, 17].
In addition, the primary alloantigen response provided a fortuitously optimal dynamic range of response for the bivariate analyses of CCL4 synthesis and HIV-1 infection monitored by intracellular CCL4 and p24 expression.
The homogeneous diffuse light, together with the calibration steps, ensures an optimal dynamic range and minimizes shadows and shading effects as well as specular reflection and gloss-related effects.
Detector sensitivity and gain were set to achieve the optimal dynamic range of detection.
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Under optimal conditions, the dynamic range of this DNA biosensor for detecting the sequence-specific DNA of phosphoenolpyruvate carboxylase (PEPCase) gene from transgenically modified rape was from 1.0 × 10−13 to 1.0 × 10−7 mol/L, and the detection limit was 2.1 × 10−14 mol/L.
Any experimental results shown in this paper are meant to illustrate the unique prowess of the FF2-6-001 qPCR set-up tool and to aid in discussing the concepts of qPCR inhibition and optimal qPCR target dynamic range; they are not intended to represent a complete scientific study per se.
Images of positive staining were adjusted to make optimal use of the dynamic range of detection.
The ECL Western Blotting Detection kit (GE Healthcare, Buckinghamshire, UK) was used to obtain a chemiluminescence signal, which was then detected using Amersham Hyperfilm ECL (GE Healthcare, Buckinghamshire, UK) at varying exposure times to obtain images with optimal density within the dynamic range of the film (30 s–30 min).
Treatment with doxycycline at 2 µg/ml for 48 h was considered optimal, providing a useful dynamic range of neuroserpin expression.
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