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Response surface methodology (RSM), including central composite design, was used to determine the optimal concentrations and conditions.
Following a PBS wash, primary antibodies were added at previously determined optimal concentrations and left overnight in a humidified chamber at 4°C.
Onto this primary mixture, L-lactic acid was first added followed by the other aliphatic carboxylic acids initially one at a time to determine their optimal concentrations, and then jointly at their optimal concentrations to create the final blend.
Samples were assayed at optimal concentrations and according to the manufacturer's instructions.
Further studies are required to identify the most efficient plasminogen activators and FSDs, their optimal concentrations, and the appropriate application strategies.
Cells must maintain optimal concentrations and speciation of copper by tightly regulating the uptake, distribution, storage, mobility, and efflux of this ion.
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The optimal concentration and time of vanadate addition was 50 μM and on day 4, respectively.
qPCR reactions were performed to determine primers' optimal concentration and PCR efficiency.
Pickling pretreatment with a mixture of these acids at an optimal concentration and an optimal pickling time resulted in relatively uniform dissolution of the alloy surface.
It is shown that there is an optimal concentration and number of AuNPs layer that can effectively enhance the sensitivity of a label-free capacitive immunosensor system.
Central composite RSMD experimentation predicted that the optimal concentration and ratios of these inorganic compounds was 1.04% NaNO2, 1.07% Na2S2O3 and 0.98% Na2S.
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