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Finally, by using DF, the responses were optimized simultaneously and the optimal concentration was located to be 73 g/L sucrose, 11 g/L yeast extract, 8 g/L peptone, and 46 mM Mg2+.
The optimal concentration was determined.
The optimal concentration was found to be at 10%Na2CO3O3.
This optimal concentration was tested for UV/H2O2/Fe3+ process; ranging [Fe3+]: 2–20 mg·l−1.
The concentration of phosphoric acid was also well tuned and the optimal concentration was 2.5 mol L−1.
The amount of sludge generation was in the range of 100 175 mg L−1 and the sludge produced at the optimal concentration was 170 mg L−1.
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The optimal concentration is generally high and represents a balance between mobility gains and injectivity losses.
The optimal concentration is reached since 50 μg ml 1.
Results obtained with the optimal concentration were plotted relative to negative (0.1% DMSO) and positive controls (RO2949097, 5 μM).
Figure 4c shows that the best reaction conditions were observed at values between 6 and 50 mM, with an optimal concentration being around 20 mM NaCl.
Cell surface staining with antibodies against CD3, CD4 and CD8 added in FACS buffer (PBS with 1% BSA and 0.05% sodium azide) at predetermined optimal concentrations was performed before fixation of cells for 15 min with 4% paraformaldehyde.
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