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The optimal cell concentration was 0.05 g/mL in hypertonic solution and 0.2 g/mL in hypotonic solution.
When the optimal cell concentration for single cell patterning (1.0×105 cells/ml) was used, the patterned cell viability was 97.0±0.8%.
The growth curve was performed in order to evaluate the expansion and replication abilities, standardize the optimal cell concentration for cell growth, and assess their kinetic behavior.
To achieve a suitable occupancy frequency of one cell per well, we identified optimal cell concentration by plating different cell densities into Terasaki plates using a principle similar to limiting dilution cloning [ 18].
In order to underline these phenomena, we reckoned that 10 cells/well in a final culture environment volume of 200 μL would be the optimal cell concentration necessary to assess the cytotoxic potential of the peptides.
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The standardization of Vero cell culture in serum-free medium for in vitro T. gondii tachyzoite production was performed establishing the optimal initial cell concentration for the confluent monolayer formation, which was 1 × 106 Vero cell culture as initial inoculum.
The optimal conditions of cell concentration, agar agar concentration and bead size were 7.8 g/L, 5% (w/v) and 6.2 mm.
These results suggest that the scale-up was successful and there is potential for further improvement using optimal C/N ratio and cell concentration values.
While for single side illumination condition, an optimal combination of the initial cell concentration, light intensity, and reactor width may have to be considered for high H2 production.
A full factorial design was used to obtain the optimal LAB/PNSB ratios and initial cell concentration for hydrogen production by bioaugmentation system.
These minerals are essential to all yeast and must be present in millimolar concentrations for optimal cell growth [ 22].
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