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Optimal bindings were seen at concentrations of 100 to 200 pmol/50 µl of the scBsDb (data not shown).

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The peptides designed for optimal binding were prepared by combining preferred amino acids at each position.

Optimal binding was seen when the yeast cells were contacted with jute fabric treated with 2% polyethylenimine.

The optimal binding was obtained with a (GlcNAc 5.

In terms of probe design it appears that optimal binding is obtained if the fluorophore is conjugated to N1 of granisetron via a short (butyl) aliphatic spacer.

The optimal binding ratios were between 2 to 1 and 1 to 2. Using the l-l-GFP-l linker peptide we were able to recover between 78 and 95% of the maternal antibody binding (Figs. 4(d);5A,B).

The optimal binding conditions were determined by titrating GST-Ubl (1-76) 0–300 nM against Parkin 80-465 -biotinn 80-465 -biotin

For band shift assays the optimal binding conditions were determined according to the Odyssey infrared EMSA kit (Licor).

Given that these studies have all agreed that the central optimal binding motif is 5'-UUAUUUAUU-3', it is of interest to compare this sequence with the naturally occurring physiological targets of TTP found in the TNF-α and GM-CSF mRNAs.

However, enzymatic assays have shown that these inhibitors are strongly pH dependent as their optimal binding affinities are considerably diminished under neutral/alkaline conditions [ 16].

Then, this hypothetical optimal binding partner was mutated stepwise as follows: A random position in the sequence was chosen to be substituted with 75% probability, deleted with 17% or a nucleotide inserted (8%).

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