Exact(8)
In the standard reaction condition (5 mM CaCl2) the enzyme was activated after incubation for 30 min and the optimal activity was retained for up to 2 h.
Optimal activity was achieved at 60 °C for H192P compared to 55 °C for wild type.
Optimal activity was observed upon removal of the pyrimidine C-4 chloro substituent.
The catalyst activity depended on the pyrolysis temperature, where the optimal activity was achieved by pyrolysis at 1073 1123 K.
On pNPX, optimal activity was achieved at pH 6 and 40 °C and Km and Kcat values were 2.92 mM and 1.32 seg−1, respectively.
Based on the application of a global desirability analysis on all the group of enzymes detected in the multienzyme solution, an overall optimal activity was observed at 53 °C and pH 4.7.
The result shows that the optimal activity was found for 7-μm-long TiO2 tubes, and tubes longer than 7 μm have thinner walls; thus, light is absorbed on a longer distance and pollutant has to diffuse further to reach the oxidizing species [30].
The optimal activity was at pH 6.4 and 70°C.
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