OGF (10 mg/kg), the opioid antagonist naltrexone (NTX; 30 mg/kg), or saline solution (0.2 mL) was injected intraperitoneally daily for 28 days into the rats, and restenosis of the carotid artery was examined with morphometric analysis using Optimas software.
Occlusal surface areas of cheek teeth were measured from digitized pictures using Optimas software.
Time-lapse image series were captured and stored on a computer hard drive at 30 seconds intervals with a JAI CV-M10 CCD camera and an Imagenation PX610 frame grabber (Imagenation Corp., Beaverton, OR) controlled through Optimas software (Optimas Corp., Bothell, Washington).
Briefly, gray-scale digitized images were imported into the Optimas software (Optimas 6.0, Optimus Corp., Bothell, WA, USA).
Briefly, gray-scale digitized images were imported into the Optimas software (Optimas 6.0; Optimus Corp., Bothell, Washington, USA).
SP-positive nerve fibers were identified by segmentation using threshold gray levels with the Optimas software.
A series of images representing all of the tracheal smooth muscle in a section were collected in digital files, saved to an internal database, and measured digitally with Optimas software (Media Cybernetics Inc., Bethesda, MD).
Morphometric analysis were performed on selected sections stained for distinct markers using an automatic image analyser employing Bioscan Optimas software (Optimas, Edmonds, CA, USA, Version 4.10) on a total of 50 glomeruli per animal; in a minority of samples with not enough glomeruli, cells were counted in at least 20 glomeruli.
Images of the DG processed for lectin histochemistry were obtained with a Panasonic GP-KR222 CCD camera on an Olympus BH-2 microscope at 40× magnification and analyzed using Optimas 6.5 software.
