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The method was performed as described in Moore et al. [10] with 2 minor modifications; 48 well plates were used in place of 24 and a plate sealer (Biorad optical tape, Biorad, Hercules CA, USA) was used to avoid evaporation and cross-contamination in the plate.
The plates were sealed with optical tape for the experiments.
The reactions were set up in 96-well plates covered with optical tape (Genesee Scientific).
All assays were carried out in 96-well plates, which were covered with optical tape.
All assays were carried out in 96-well plates which were covered with optical tape, as described [ 13, 46].
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However, irrespective of luminometers, the adhesive optical tapes typically used to seal plates failed to maintain a proper seal after two days of data collection, introducing significant well-to-well variation in bioluminescence and/or cell survival.
The assays were performed in 96 multi-well PCR plates covered with optical tapes in the Applied Biosystems 7500 Real-time PCR System in a final volume of 25 μl, containing 10 ng of cDNA, 12.5 μl of Power Master Mix 2X Applied Biosystemss), 5 μmoles of either HPSE, Matrix Metalloproteinases (MMPs) or GAPDH forward and reverse primers, and water.
Each reaction was done in duplicate in a volume of 20 µL with 96-well optical-grade PCR plates, sealed with optical sealing tape (Bio-Rad).
A database is stored as a file or a set of files on magnetic disk or tape, optical disk, or some other secondary storage device.
Right and left Full HD video streams of the twin-lens 3D camcorder can be recorded as files on SDHC/SD Memory Cards, ensuring higher reliability than on other tape, optical disc, HDD or other mechanical-based recording systems.
The PCR plates were sealed with optical quality sealing tape (Bio-Rad).
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