Structured fluorescence image pairs were acquired at 200 Hz and processed by DIFF optical sectioning to obtain DIFF-sectioned images at 100 Hz.
Optical sectioning by DIFF and HiLo.
Mertz, J. Optical sectioning microscopy with planar or structured illumination.
This implies an intrinsic 3D optical sectioning effect.
Noguchi, A., Ishiwata, H., Itoh, M. & Yatagai, T. Optical sectioning in differential interference contrast microscopy.
It was twenty years ago today: a celebration of optical sectioning.
Essentially, the optical sectioning picture breaks down when approaching the fundamental depth limit.
Optical sectioning microscopy can provide highly detailed three dimensional (3D) images of biological samples.
If in our system we had used shifted sinusoids, we could have performed both optical sectioning and descattering.
Confocal microscopy has allowed optical sectioning and reconstruction of tissues in three dimensions.
In those scenes the system will perform descattering (as defined in observation #3) but not optical sectioning.
