Sentence examples for optical sealing from inspiring English sources

Each reaction was done in duplicate in a volume of 20 µL with 96-well optical-grade PCR plates, sealed with optical sealing tape (Bio-Rad).

qPCR was performed using an Applied Biosystems 7300 Real Time PCR System in an optical 96-well plate covered with optical sealing tape (Applied Biosystems, Foster City, California, USA).

Plates were sealed with optical sealing film and subjected to thermal cycling in PCR 9700 thermal cyclers (Applied Biosystems) at 95°C for 15 min, followed by 40 cycles of 92°C for 15 sec and 60°C for 1 min. Following thermal-cycling, plates were assayed for fluorescence using a 7900HT sequence detection system (Applied Biosystems).

The PCR plates were sealed with optical quality sealing tape (Bio-Rad).

Briefly, using a 96-well PCR plate, SYPRO Orange was mixed with protein in 1∶1000 ratio with final volume of 20µl/well (triplicates/sample and with/without EDTA at 11µM and 33µM) and sealed with an Optical PCR seal.

One µl of cDNA product was diluted 1∶2, and 2 µL was amplified in a 20 µL reaction mixture containing 12.5 µL of SYBR Green Supermix (Bio-Rad) and 0.4 µM each of forward and reverse gene-specific primers (Supplementary data, Table S1), aliquoted into 96-well, 0.2-mm thin-wall PCR plates, and covered with optical-quality sealing tape.

Reactions were loaded on 96-well thin-wall plates and sealed with optical-quality sealing tape.

Real time PCR was performed on a 7900 HT Fast Real-time PCR system using 96-well optical plates and sealing film (Applied Biosystems Inc., Foster City, CA).

At this argon/methane ratio, the carbon coating has the best ability to minimize low temperature induced delamination or microcracks, so is the best for the production of a hermetically sealed optical fiber coating.

To prevent cross-contamination from evaporation, plate seals (optical flims, Biorad) were used.

The reaction mixture was finally transferred into a 96-well optical reaction plate, sealed with appropriate optical caps, and run on the ABI Prism 7700 HT apparatus (Applied Biosystems).