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These color-changing properties provide a simple and powerful technique for regional optical marking (Ando et al. 2002).
The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking.
These properties allow a tightly controlled switching using comparably low irradiation energy, and are beneficial for local optical marking and super-resolution microscopy.
The possibility to control the fluorescence properties enabled numerous innovative applications, for instance, regional optical marking and tracking of cells, subcellular organelles or proteins [3], [15], [21].
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This optical marking-off system was much more economical in terms of space and skilled labour than the older method.
The director was equipped with optical sights, optical Mark 45 Rangefinder (the long thin boxes sticking out each side), and a Mark 13 Fire Control Radar antenna (the rectangular shape sitting on top).
Surveys were administered electronically at PUGSOM and JHUSOM and given on paper, using optical mark reader sheets, at CUCMS.
Fairly recently, optical mark-recognition systems have been developed to enhance the transfer of the data into databases.
The scanned data are 'captured' using computerized intelligent character recognition and optical mark reading software (Eyes and Hands®; Readsoft Inc, Slough, UK).
Hand search data were recorded on a paper based data collection form that was compatible with an optical mark and character recognition system called Teleform (Cardiff Software Publishing, Bozeman MT).
The questionnaires are gathered and encoded on optical mark recognition (OMR) cards by a trained clinical research center (CRC) nurse at each research site.
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