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Placing polystyrene particles with a refractive index of 1.59 and 3μm radius at a grid of points we produced and stored a map of the optical force efficiency.
As can be seen in Fig. 2(a), the optical force efficiency is highest at around z = 16.5μm at a lateral distance of about 2.5μm away.
The stirring effect saturates at higher laser powers since the order of the optical force efficiency drops rapidly after 3μm, as was shown in Fig. 2(b).
The units of optical force efficiency Q, can be related to the optical force, F, by F = nPQ/ c in which P is the beam power at the focus, and c is the speed of light in free space.
The calculated optical force efficiency, Q, in the x-z plane is shown in Fig. 2. As previously reported, the transition from pulling to pushing occurs at some distance above the focal point of the laser beam [ 17], which in this case is at 13μm.
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Sharp contrasts often underscore the sheer optical force art can muster.
For our optical force calculations, we focus on the strong optical response spectrally locating at ~793 nm (Fig. 1c).
These curves in Fig. 8 indicate that the performance of the optical force is attraction; the optical force drives SNP toward SI.
Force measurements with optical tweezers require test beads to be in the harmonic potential well of the trapping optical force and to be displaced from its equilibrium position inside the well only by the force to be measured.
The net optical force F optical on a cell surface can be written in this form: F o p t i c a l = n m P Q c, (2 where n m is the buffer medium index of refraction, P the total laser power, Q the dimensionless trapping efficiency and c the speed of light in vacuum.
Based on this observation, we safely approximate the optical force at points beyond 8μm as zero.
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