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To induce T7 RNA polymerase activity besides E. coli RNA polymerase activity the main culture was induced with 0.5 mM IPTG an optical density measured at 600 nm (OD600) of 0.5 0.6.
They show a good long time cyclic stability, but the change of the optical density measured at 550 nm after 25 000 cycles was only 0.13.
optical density measured at a wavelength of 600 nm.
Optical density measured at 600 nm (OD600) was measured simultaneously.
where OD is the optical density measured at a given layer thickness t.
where OD is the optical density measured for a given film thickness (t).
Similar(13)
Figure 8 shows the comparison of optical densities measured from the WST-1 assay results.
Optical densities measured at dilutions of anti-AtxA IgG of 1/16,000 were used for cross-reactivity gradation: level 0: OD≤0.1, level 1: 0.1<OD≤0.2; level 2: 0.2<OD≤0.3; level 3: 0.3<OD≤0.4; level 4: OD>0.4.
The ratio of the optical densities measured at 260 and 280 nm were >1.9 for all RNA samples.
Results were expressed as percentages of the ratio between the optical density values measured in supernatants and the total optical density value measured in cells plus supernatants.
The number of bacteria was deduced of the optical density measure at 600 nm.
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