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U87MG, MDA-MB-435, A549, HT29, and PC-3 tumors were harvested from the tumor-bearing mice, and were immediately snap-frozen in OCT (optical cutting temperature) solution, and then cut into slices (5 μm).
The U87MG tumors were immediately snap-frozen in the OCT (optical cutting temperature) solution, and were then cut into slices (5 μm).
The larvae were anesthetized in CO2, directly embedded and shock-frozen in optical cutting temperature compound (OCT; Sakura Finetec, Staufen, Germany).
Formalin fixed, optical cutting temperature embedded myocardial samples tissue sections of 8 μm thickness were washed with distilled water and PBS, and subsequently exposed to a picro-sirius red solution (0.001% (w/v) sirius red in saturated aqueous picric acid) for 1 hour.
Explants were embedded in optical cutting temperature (OCT) and snap-frozen in liquid nitrogen prior to cryosection; and 10-μm sections were obtained and embedded on glass slides, fixed with 4% paraformaldehyde (diluted with PBS) for 30 minutes at 4°C, followed by washing three times with PBS for 5 minutes.
Tumour tissues were embedded in Optical Cutting Temperature compound (Tissue-Tek, Sakura Finetek).
Similar(51)
film, relying solely on optical effects, cutting negative, and printing on film (as, for instance, Anna Biller did in "The Love Witch"), rather than making a film that is itself created with digital effects.
In the nineteen-eighties, the Archives transferred some two hundred thousand documents and images onto optical disks — the cutting edge of new technology at the time.
The structural strength of the whole thin diaphragm based sensing probe is improved by the ingenious design, and a high precision optical fiber cutting system can be used to control the length of air micro-cavity.
The double-layer coatings were characterized by X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), in-depth glow discharge optical emission spectrometry, and cutting tests.
Until firms such as BellSouth start boosting their capital-spending budgets rather than cutting them, optical networking firms such as Ciena will have a hard time posting results that please Wall Street.
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