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First, the S-TOP signals are quantitatively analyzed with a newly developed 3D computational ray tracing method; the simulator can trace enormous ray segment trajectories in an optical fiber and render complicated optical boundary conditions.
Next, a similar mask of the alveolar airspaces was made, a grid of 42 points was placed on the mask, and the points overlaying the airspaces were counted; truncation of airspaces by the optical boundary was ignored.
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(b Lesion delineation to define the optical boundaries of each lesion in TLI imaging: the region of interest (ROI) was superimposed on the perfusion image, and the perfusion parameters are calculated.
Polystyrene phantoms consist of discrete monodisperesed spherical particles suspended in water with significant scatterer/background refractive index contrast (~1.59/1.33) while biological tissues, like liver, are random-continuum type of media (connective fibers, cells, nuclei, other cytoplasmic organelles) without distinct optical boundaries and much lower index contrast variations [ 38].
The precipitating particle data are used to validate the optical auroral poleward boundary location along the satellite tracks.
As already described, nuclear boundary optical density and Ab threshold were adjusted for each case examined [ 15].
Their result is explained in terms of zone-boundary optical phonon emission from high energy electrons.
The two distinct microstructural features, as observed by polarized light optical microscopy, were grain boundary serrations (GBS) and in-grain inclined lines (IIL), typically observed at an approximate angle of 35° with rolling direction (RD).
The aim of fluorescence tomography is to obtain the distribution of a fluorophore inside an object (an only indirectly accessible quantity) from optical measurements at the boundary (an easily obtainable information).
A multimode fiber based illumination system was also combined with this integrated system to allow the efficient visual identification of the cell boundaries during optical transfection.
This means that it will be possible to use the probe on a variety of tissues or at organ boundaries where optical properties may vary significantly.
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