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Unlike traditional histological (found to be generally reliable, but destructive) and biochemical (non-invasive, but found to be unreliable) tissue analyses, label-free optical assessments had the advantages of being both non-invasive and reliable.
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The optical assessment allowed a spatial resolution in a 2D field of view around 120 μm/pixel.
Surface properties resulting from laser irradiation were evaluated mainly based on surface morphology, chemical alterations and general optical assessment of deposited copper layers.
Fatigue related damage growth without feasibility of optical assessment can be monitored conveniently by means of the direct current potential drop method in laboratory experiments.
For optical assessment by flow cytometry, cells were additionally stained with CD14 and CD16.
For optical assessment a Near-infrared fluorescent (NIRF) fluorochrome (VT680, Excitation 670±5 nm, Emission 688±5 nm, Visen Medical, Woburn, MA) was coupled to cross-linked iron oxide nanoparicles (CLIO) as previously described [33].
Alternative optical assessment techniques have been investigated with the goal of reducing the number of excised nodes.
The conventional optical assessment of platelet aggregation by Born is technically demanding, expensive and requires platelet-rich plasma (PRP) [ 14].
There are several possible limitations to our study with respect to the optical assessment of breast cancer.
Future studies will incorporate these patient demographic variables into the optical assessment to determine which parameters would be best utilized for margin assessment.
Prior to the MR examination all patients underwent a clinical investigation, including optical assessment and an intraoral bitewing X-ray of the suspicious areas.
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