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To test cell viability, the number of DAPI positive nuclei and neurons was counted from 10 optic fields for each condition.
Beginning with the 6× concentration, the standard-deviation becomes larger, meaning that there were already some optic fields per/coverslip, where cell viability was reduced while in other regions cells remained healthy.
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Appropriate design of optical paths (Fig. 2b) not only provided homogeneous optic field but also shortened the focusing distance, which helped us get the high-quality images within just 20-mm height shell.
This process begins with the differentiation of cells that form the optic field and ends when the optic cup has attained its shape.
Every third section of a region was subdivided into fields where each field was equivalent to the microscopic optic field at 40x.
Viability is given as the percent of cells that are unstained by PI over the total number of cells in the optic field.
The number of cells migrating through the filter was counted and plotted as the number of migrating cells per optic field (×10).
One optic field covering ≥100 cells per central brain hemisphere from each brain was included; samples were collected from two to three independent aging crosses.
The number of invading cells through the filter was counted after H&E staining and plotted as the mean number of invading cells per optic field in three independent experiments.
Films were then analysed and the number of cells migrating into the cell-free zone created by the scratch of area 1.0±0.12 mm in width by 2.0 mm in length (corresponding exactly with the microscope optic field-of-view) were counted manually at intervals of 4, 8, 12, 16, 20 and 24 h.
Lee, KF, Reil, F, Bali, S, Wax, A, and Thomas, JE. "Heterodyne measurement of Wigner distributions for classical optical fields". Optics Letters 24, no.
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