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Structurally, the side chain of position P2 forms contacts with the side chain of position P4′ (I94) located on the opposite side of the loop.
It is interesting to note that Tt1808 has the S-PD-EXR-K variant of the active site, and that the side chain of the R118 residue, which replaced the "classical" catalytic Lys, points away from other catalytic residues, on the opposite side of the loop between the "EXR" and "K" elements.
Then, squish the ends down to the opposite side of the loop, thus making a bow.
Similar(57)
Our structure also reveals that the inhibitor approaches the active-site cleft from the opposite side of the crossover loop as compared to the direction of approach of ubiquitin's C-terminal tail, thereby occupying the P1′ (leaving group) site, a binding site perhaps used by the unknown C-terminal extension of ubiquitin in the actual in vivo substrate(s) of UCHL1.
The ability to form stable out-of-phase (two binding sites are on the opposite side of the DNA) loops with only a small reduction in stability is consistent with previous studies [26].
This joint effect is intriguing because I28 does not directly contact substrate; rather, it is on the opposite side of the WW domain loop mediating substrate binding, Loop I (S16 R21).
On the opposite side of the groove, in the loop connecting helix α3 and strand β5, only small backbone motions are seen but here Phe213 flips its aromatic ring 180 degrees towards the backbone of the loop (Fig. 4D).
Grip the wire just after the bend with round nosed pliers and bend the wire around to bring it flat against the opposite side of the pliers, forming a loop.
Put the hook point through the loop on the opposite side of the fish.
On the opposite side of the hill next to Teal Lake Bluff, the business loop intersects Arch Street, which carries traffic to Negaunee High School to the west or the football field complex to the east.
Computer modeling of the DNA trajectories suggests that the most inactive promoters have short spacer DNA and the NtrC sites on the opposite side of the helix as the wild-type sites; otherwise, the loop can form effectively.
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Justyna Jupowicz-Kozak
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