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From the recombinants, nine were positive for S8 and negative for S10 and 15 showed the opposite score.
They strongly agreed with current policies on EMFs (statement 29 note that early warners have an opposite score on this statement) and thought that legislation and regulation is the best way to manage the possible health problems concerning EMFs (statement 4).
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Another quarter saw the opposite: Scores increased before SIG, then dropped.
The opposite scoring was given for meat and dairy.
It also lists per category of agreement the number of opposite scores assigned in one or more of the other readings.
Given the very poor reproducibility at the individual joint level, we investigated whether opposite scores were being assigned to joints in independent readings (table 4).
This hypothesis is supported by our observation that opposite scores are actually very rare (table 4); opposite scores occur at a frequency of approximately 4% or less, which is close to the average percentage of joints with change across readings, and as such are most probably due to chance occurrences (differences in judgement).
When averaging over signed T-scores equal but opposite scores will tend to cancel, resulting in no module activity, when in fact there are genes significantly over or under expressed.
Reader 1 was more willing to accept patients with (a low number of) opposite scores than reader 2. But regardless of the reading or the reader, the greater majority of patients with a zero sum score were assigned "no change" to all joints, or, in case of change, a unidirectional pattern of change.
For repressed genes, we applied the opposite, a score of 1 was assigned if the expression level was less than the mean expression value for a sample to be inflammatory or was more than the mean value for a sample to be anti-inflammatory.
Interactions of aligned charges were summed over the whole overlap region of the two chains, with unlike charges (+1 opposite −1) scoring −1 and like charges (−1 with −1 or +1 with +1) scoring +1 (i.e. charges were multiplied).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com