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In the pMOD601 vector, a DsRed2 reporter gene under regulation of the ompB promoter was placed in opposite head to tail orientation to the CAT gene under regulation of the ompA promoter, with a single ompA terminator located between the 3' ends of the CAT and DsRed2 genes [39].
They were mirrored horizontally in order to generate opposite head gaze directions.
The mean preoperative head turn was 27.4 ± 5.8° (range 20 35) with mean postoperative head turn of 7.2 ± 6.6° (range −8 to 10," - indicates the opposite head direction).
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10 Emerge on to a lane and take the signed footpath opposite, heading up the field with the hedge on your left until you meet a road.
Links with opposite heading to the trace point are discarded in order to avoid matches with roads running in the opposite direction.
Afterwards, candidates are filtered using the same rules applied in the first method (maximum distance of 20 m, opposite heading and one link per super-link).
Consistent with previous observations, the majority of the newly-predicted genes are located in Abpa- Abpbg pairs, and are likely to be transcribed in opposite, head-to-head (i.e. 5'-5') arrangements.
In Drosophilids, mα is located close to mβ and is transcribed in the opposite direction (head to head; Fig. 1A).
Murine CrAT is encoded by a single gene located in the opposite orientation head to head to the PPP2R4 gene, sharing a very condensed bi-directional promoter.
We managed to get the name and details of the Selfridges buyer and we waited for five hours opposite their head office in a small cafe in anticipation of her coming out that day, but she never did.
For the opposite extreme, head to the middle of the island, to the marble quarries of Marathi — an oasis of snowy white stone that makes the whole trip worthwhile.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com