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Across the presumptive tarsus, lines modulates the opposing expression landscapes of the leg gap gene dachshund (dac) and the tarsal PD genes, bric-a-brac 2 (bab), apterous (ap) and BarH1 (Bar).
Interestingly, a third subset of genes exhibits opposing expression in between hiPSCs and donor cells.
This is based on the fact that primary AFCs and AFiPSCs and ESCs exhibit opposing expression patterns related to a large number of senescence-associated genes.
Likewise, opposing expression patterns in the population effects are observed for proteins involved in protein biosynthesis/folding/degradation: Ribosome Small subunit, Ribosome large subunit, Translation, Protein Chaperones, Protein disulphide isomerase and Proteosome (Fig. 5b).
GFP reporters of trx-1 and daf-28 show opposing expression patterns in dauers (i.e. trx-1 is up-regulated and daf-28 is down-regulated), an effect that is not observed in growing L2/L3 larvae.
The opposing expression change (E2 vs. E3, wild type) in these glycolysis and gluconeogenesis components were also highly correlated with their transcription profiles during the diauxic shift, the switch from anaerobic growth to aerobic respiration upon depletion of glucose [9], [22].
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Northern analysis performed with extracts obtained from ammonium-ethanol grown cultures, showed that BAT1 and BAT2 displayed opposed expression profiles; BAT1 expression was five-fold repressed, while that of BAT2 was two-fold increased on ethanol as compared to those found on glucose.
However, whether opposed expression levels of novel_mir_006-3p and its target gene in the CMS anthers leads to a significant increase in PCD remains to be studied.
These two transcripts, however, showed diametrically opposed expression levels and very huge fold change which cannot be explained simply by means of an additivity model (Table 4).
Our data indicate that the DAF-2 regulated transcription factor DAF-16/FOXO positively modulates hif-1 transcription under anoxia but opposes expression of the HIF-1 responsive globin genes itself.
In addition, whole-genome gene expression analysis revealed a highly differentially and opposing gene expression pattern in response to treatment with the two isoforms of CRP.
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