Sentence examples for operator element from inspiring English sources

The difference in phasing arises because the LB conformation involves a 180-degree rotation of one operator element about the sequence dyad with respect to the WA and WT conformations.

The DLD1Tr21 cell line is a human colon cancer cell line expressing high levels of the tetracycline repressor protein, resulting in a tight control of the expression of the gene of interest under control of a responsive tetracycline operator element [21].

Furthermore, the expression of the tyrosinase gene could not be controlled in recombinant DH5α because DH5α does not have the repressor protein LacIq and enables constitutive expression of tyrosinase gene from the T5 promoter / lac operator element in pMFvpt.

The de novo designed promoter including tetracycline operator elements was inactivated by the repressor protein, and inducer addition successfully initiated target protein expression.

The reason for this is the presence of one of two particularly inefficient operator elements: L2 at the upstream position (F2, F6, F26) and the presence of a single λ operator at the downstream position (F10, F11, F14).

Using a combinatorial synthesis approach [9], we shuffled multiple operator elements within the promoter region (Fig. 1A,B) to create complex regulatory architectures capable of executing diverse promoter-encoded logic (PEL) functions.

For those that fail to obey this code, the leakiness of the states that fail to conform to the expected logic type can be traced again to two operator elements, single λ operators downstream and upstream (M12, M20, M21) and single L2 operators upstream (M17).

In light of these predictions, operon structures can be estimated and based upon them further sequence regions can be derived with high probability of contained promoter and operator elements.

The expression vector contains two lac operator elements which permits in combination with high levels of lac repressor pro-vided from the pREP4 plasmid, some control over the expression on the transcriptional level.

pAdCR5-LacZ-AgeI was digested with HindIII to remove the operator elements (6X-CuO) and the CMV5 PCR fragment was cloned as a HindIII fragment to generate pAdCMV5LacZ-AgeI.

Note that all mutants share the same set of users (U), roles (R), permissions (Pr) and inputs (I) of the original policy P. The set ( mathcal {R}^{P}_{fault}) of faulty policies is generated by making changes using two kinds of operators: mutation operators and element modification operators.