We monitored the localization of a native telomere in living cells by microscopy, using a strain that has telomere VIR tagged with an array of Lac operators bound by green fluorescent protein (GFP -LacI and telomeric LexA operators.
Expression of this operon is induced by the presence of camphor, because a TetR-homolog transcription factor, CamR, dissociates from the bound operator when bound to the camphor ligand.
This effect raises the occupancy of the primary operator, the fraction of primary operator sequences bound by LacR, from 0.79 to between 0.985 and 0.998, a value essentially insensitive to helical phasing (Figure 7, upper panel).
begin{aligned} e.g.,~data_partitioned = small {text {PARTITION}}~ data_items~ small {text {BY}}~f1; end{aligned} BIND: Contrary to the PARTITION operator, BIND operator combines two or more streams into one.
I argue that an adequate first-order approach to conditional logic must admit domains that vary across possible worlds; as well the most natural expression of the conditional operator binds variables (although this binding may be eliminated by definition).
In E. coli, the lac operator binds lac repressor at two sites in inverted orientation.
We marked CEN5 by the adjacent insertion of a tet operator array bound by TetR-3CFP (cyan fluorescent protein) and CEN15 by a lac operator array bound by GFP-lacI [21].
Again, we assume that this process is very fast, so that V, the fraction of time during which a particular operator is bound by a particular TF, in either apo or ligand-bound form, is simply the function of the current concentration of all the TFs which can bind this operator.
Cohesion of the resulting circular DNAs was then measured cytologically using operator arrays bound by repressor proteins fused to GFP.
V is the fraction of time that an operator is bound by one particular TF out of all possible TFs with a corresponding binding sequence (no).
We derive a pointwise estimate on |pt x, y)− pt x, y)| as well as anLp-operator norm bound, wherep∈[1, ∞], forPt−Ptin terms of the localL2-distance betweenaijandãij.
