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Single chain TetR variants were constructed that contain DNA reading heads with two different operator binding specificities.
Conformational plasticity of the coiled-coil domain of BmrR is required for bmr operator binding: the structure of unliganded BmrR.
In all instances where two operator binding sites for a given TR are present, the repressors always manage to effectively bind and outcompete RNAP binding.
The synthetic transcription factor reporters consist of a constitutive promoter followed by the operator binding site for the transcription factor of interest.
It is relatively simple to develop synthetic reporters for intercellular concentrations of effectors by cloning a library of reporters with degenerate operator binding sites [ 43].
Examination of the intergenic region between abyC and abyD revealed a 16 bp perfect palindromic repeat sequence, TGAACTGATCAGTTCA, that is likely to be the operator binding sequence for AbyC.
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Treatments of lysogenic cells with mutagenic concentrations of ethanol did not trigger prophage induction and were concluded not to induce a cellular SOS response nor to denature the prophage repressor, or to disrupt repressor-operator binding.
One or more of these structures could function as operators, binding of ParG to one or both of these two structures may result in inhibition of transcription of parF and parG.
Ligand-TF and TF-operator binding are assumed to be fast processes and set to quasi steady state.
To overcome these hurdles, we designed a conditional constitutive expression system equipped with the artificial transcription factor, AraCC, which has two operator-binding domains and simultaneously binds to the I1 and I2 operators of the PBAD promoter for gene expression in an arabinose-independent manner.
I argue that an adequate first-order approach to conditional logic must admit domains that vary across possible worlds; as well the most natural expression of the conditional operator binds variables (although this binding may be eliminated by definition).
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