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Although the HEK293T cells were ideal for the flow cytometry-based screen due to their generally high transfection efficiencies and comparatively stable DNA content, for subsequent more directed cell cycle experiments MRC5 primary fibroblasts [21] and the U2OS osteosarcoma cell line were used because they have comparatively more operational checkpoint machinery.
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"T.S.A. completed comprehensive operational testing and evaluation of this technology and is confident that it will significantly increase our detection capability at the checkpoint".
To develop a robust method for both growth control and specific productivity improvement by avoiding pleiotropic cellular responses and complicated operational procedures, we attempted to identify an approach that results in exclusive G0/G1 arrest by directly targeting the cell cycle G1 checkpoint with small molecule compound.
Checkpoints exist at G1/S, S, G2/M and M in the cell cycle, and all these appear to be operational in Brca2 mutant cells [ 38**, 51**].
A checkpoint.
Opportunity is still operational.
"They're not operational".
"They were operational engineers".
It's more operational.
I pass a police checkpoint.
Traffic slowed near the checkpoint.
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