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Generally, economic studies suggest less than full compensation rates will encourage positive behaviours, but the non-economic literature indicate significant variation in response to compensation reflecting heterogeneity of livestock keepers in terms of their values, goals, risk attitudes, size of operation, animal species and production chain characteristics.
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At 48 h following operation, animals were harvested for isolated heart protocol using the Langendorff suspension.
Additionally, LVESD, LVEDD and LVEDP were both higher in operation animals compared to the other two groups, while no differences were seen among the three groups in IVSd or LVPWT (data not shown).
After operation animals have been allowed to move freely.
After the end of the operation, animals were transported to their cages.
56 days after operation, animals were sacrificed for biomechanical and histological analysis.
After microcirculatory measurements or sham operation animals were sacrificed in deep anaesthesia by blood samples taken from the arterial line.
Sham operation animals were subjected to the identical surgical procedures except for application of micro-aneurysm clips.
During operation, animals were positioned in a head-restraining device to allow proper access to the maxillary molar area.
Two weeks after the operation, animals were killed by cervical dislocation, hearts were removed, cleaned from fat tissue and big vessels, washed in PBS and weighed.
All surgical and post-operation animal care procedures were identical to the methodology described above.
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CEO of Professional Science Editing for Scientists @ prosciediting.com