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The standard helix opening assay (25 μl) was carried out in a buffer containing 40 mM HEPES-KOH (pH 7.5), 8 mM Magnesium acetate, 50 mM potassium glutamate, 1 μg poly dI/dC, 30% v/v glycerol, 320 μg/ml BSA and 550 ng supercoiled template (pUC_OriMtb), with indicated amounts of DnaA and or IciA (Rv1985) protein and 5.0 mM of either ATP or ADP or ATPγS (Lithium salt).
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This, along with allele switching, opens up assays that have so far been nearly impossible.
For our helix-opening assay increasing amounts of DnaA protein (0.025 0.3 μg) were incubated in presence of 5 mM ATP with supercoiled pUC_OriMtb, as described.
These data, besides demonstrating the robustness of the DASL assay, open up new possibilities for analyzing gene expression using microarray- based assays on FFPE material.
The proposed assay opens a promising platform of clinical immunoassay for other biomolecules.
This electrochemical assay opens the way for designing robust, reliable and inexpensive odorant biosensors.
This assay opens a new horizon for both qualitative and quantitative detection of TDG, holding great promise for potential application in cancer cell research and clinical diagnostics.
This GFLV detection assay opens new avenues for epidemiological studies and for molecular investigations on the mechanism of X. index-mediated GFLV transmission.
It provided the procedure for putting the coins aside, sealing them in envelopes, and placing them in a pyx to be opened by the assay commissioners.
Our assay opens the way to characterizing the ribosome's full mechano chemical cycle.
However, PLA2R ABs assay opened a new perspective: at some point, serology will replace histopathology in the diagnosis of MN.
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