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The same remark holds true for the fusions with any open sequence cloned in only one reading frame just upstream the PRIG MCS (KRAB, VP16, fluorescent protein), although in these cases the compatible restriction site has to be at the 5'end of the cDNA (see below).
One solution to this issue lies in the proper use of precursor ion mass differences between the spectral pairs to be matched; that is, the mass differences should be considered as the potential PTM masses, as done by some open sequence database search engines, e.g. PTMap (Chen et al., 2009).
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Open sequences of XIC-linked genes in human and mouse were compared to porcine genome and sequence homology of each orthologue to porcine genome was calculated.
These data would seem to indicate, therefore, that the most open sequences are correlated with early replication, in agreement with the results reported by Gilbert et al. [ 22] for the 1 Mb array markers.
The North American version of the game was playable at Electronic Entertainment Expo 2002, and IGN noted that the opening of the game did away with the notoriously boring opening sequence of Golden Sun, introducing the characters in between the action.
First, the (rather blurry) opening sequence of Terry and June.
Plus – in a beautiful drawn-out opening sequence – a brilliant grain-tombstoning game.
The "Battlestar Galactica" opening sequence is damned by "The Brady Bunch" problem.
She holds her hands coquettishly above her head, dancing like the sultry silhouette in the classic James Bond opening sequence.
Following an explosive but raggedly edited opening sequence in Nigeria, the Avengers are forced to confront the human cost of their ambitious action set pieces.
I see a Jack Black-directed opening sequence where AC/DC's Angus Young, dressed as a schoolboy, tells Sarah Palin: "No, mommy, I WON'T clean up my motherfucking room!" Then KARRRANG!
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com