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Most of open rings were hexamers and basically not transformed to the closed state (Supplementary Movie 9).
HS-AFM images of ClpB reveal highly diverse and transient oligomeric forms exhibiting round and distorted closed rings and even open rings.
On the other hand, the open rings of hyperactive Y494D mutant are expected to show dynamic conformational changes because of weak interactions among protomers.
After making histograms of circularity, the threshold to distinguish between the closed and open rings was determined from the overall distributions.
This result implies that in the Y494D mutant, the distorted closed rings are unstable and rapidly change to the open rings, which would not be able to be captured clearly in our HS-AFM observations.
The repressed E423A mutant showed both closed and open rings (Fig. 4c and e, and Supplementary Figure 16a), as with the case of ΔN-TClpB at high [ATP] (Fig. 1h and i).
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The opening end may be either easy open (ring pull) or classic (can opener needed) (Oldring & Nehring 2007).
"He has one of the most open, ringing high C's of any tenor singing today".
Surprisingly, we indicate that PKS/NRPS releases its product with an open ring structure, probably as an alcohol.
The parent isoxazole compound is converted in vitro and metabolized in vivo to an open ring isomeric form, SU0020.
An open ring feeder distribution system is considered to evaluate the performance of the proposed protection scheme.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com