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We note that while conductance assays require higher open probability for detection, dye permeability experiments are more sensitive and can detect even a small probability of opening.
We show here that this was largely due to lack of a faithful representation of the open probability for the Ca 2+ conductance of the TPC2 signaling complex.
Moreover, as already mentioned, the slower unbinding rate gives rise to longer burst durations in these channels, further increasing the overall open probability for this receptor.
The open probability for sparklets in HEK293T/17 cells was significantly less than for sparklets in isolated dorsal root ganglion neurons.
Although we did not observe any significant changes in open probability for T-LTCCs and C-LTCCs in HF, we found that T-LTCCs had ≈25% smaller amplitude in HF in comparison with control T-LTCCs.
Histogram analysis of the activity bursts gave an open probability for the wild-type KcsA of 4.8% with open current amplitudes distributed around 6 pA in agreement with earlier studies [10,23].
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This activation trend inversely correlates with the reported open probabilities for KCNQ channels [33] [36], suggesting a rationale for the varied effectiveness of PBA.
In the control extracellular solution, the open probabilities for wild-type TRPA1 were apparently below 0.5 so that values for the voltage for half-maximal activation (V50) could not be derived from steady-state currents [ 4, 29].
Accordingly, the low open probability of dSlo1A was the second reason for the flickery single-channel openings.
This phosphorylation increases the open probability of the channel [ 19], allowing for greater trans-sarcolemmal Ca2+ influx with each depolarization and producing, in part, the positive inotropic effect of epinephrine.
This phosphorylation increases the open probability of the channel [ 10], allowing for greater trans-sarcolemmal Ca2+ influx with each depolarization and producing, in part, the positive inotropic effect of Epi.
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