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Paz employs over 1,000 staff and says keeping it open costs him R$2m (around £500,000) every month.
An 18th-century sash pin with a leather strap and nail for keeping windows open costs $3.25.
In practice, Mr. Ngcobo said, most families consent to recycling only under financial duress: using someone else's grave costs 320 rand, or about $53, while acquiring a new gravesite at Red Hill Cemetery, one of the two still open, costs about $250.
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There are other avenues to consider for those looking to move to open, cost-optimized design principles that may address some of the shortcomings seen with OCP today.
We aligned all of the sequences using CLC Genomic Workbench software with the following parameters: gap open cost, 10; gap extension cost, 1; and alignment type, very accurate.
Alignments of predicted peptide sequences for each gene were performed in CLC Genomics Workbench using the Create Alignment tool (Gap open cost = 10, Gap extension cost = 1, End gap cost = Free, Alignment mode = Very accurate).
17 Parameters set in creating a sequence alignment in CLC are as follows: gap open cost, 10; gap extension cost, 1.0; and very accurate progressive alignment algorithm is used.
Opening costs for DCs are assumed as identical for all potential places.
The authors drew uniformly at random connection costs from [1000, 2000], and the facility opening costs from [100, 200].
The number of facilities, the number of customers, opening costs and assignment costs are provided in UFLP files.
Eq. (3) defines f1 as the sum of driving costs and opening costs; observe that for j ∈ V, a DC in node j is opened if ∑ k ∈ K z jk = 1.
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