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The determination of the two "open" conformation structures represents a new step towards the complete elucidation of the enzymatic mechanism of the MurD ligase.
The two PRP51-D25N dimer structures were compared with open conformation structures of PR and PR20 as well as their DRV-bound complexes.
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Electron density was observed in the ligand-binding site of the open conformation structure.
In the open conformation structure, electron density was observed in the binding site, although it could not be unambiguously assigned to glycine betaine.
The best resolved apo-open conformation structure was refined at 2.0 Å (Table 2, Figure 2A), and in this structure, only residues 139 141 were without interpretable density and were not modeled.
To fully understand the molecular basis of agonist/antagonist binding, channel activation and subtype selectivity, further P2X receptor structures, including the ligand-bound state (in closed and open conformation), and structures of different receptor subtypes (particularly the mammalian receptors) are needed.
During MD simulations of MCT1 starting from the inward open conformation, the structure becomes more compact with a kink in helix 1.
Crystallized in an inward open conformation the structure identifies a hinge-like movement within the C-terminal half of the transporter that facilitates opening of an intracellular gate controlling access to a central peptide-binding site.
The open form conformation structure of TTE-HsdS and the closed state model of M.EcoKI-M2S1 complex (Figure 2A, C) were used to construct the open state model of the M2S1 complex.
While the 1YXP structure shows the open conformation the 1ZCI structure has four continuously stacked bulged-out flanking bases (Fig. 1 C ).
Compared with differentiated somatic cells, ESCs have unique features: they have a more "open" conformation of chromatin structure, including the telomeric region.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com