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Engraving in the first half of the 17th century gradually abandoned linear clarity in favour of crosshatched chiaroscuro (shading) effects, the highlights formed by sometimes completely opaque spots.
Different concentrations of a lipase produced opaque spots with different patterns and intensities on polymer films.
The density of the opaque spots can be quantified and correlated to the enzyme concentration.
For the lipases from P. cepacia and R. arrhizus, the intensity of opaque spots decreased as the enzyme concentration decreased.
Opaque spots appeared white on the polymer film surface but the scanned picture was inverted to make the spots look dark on a white background.
In order to obtain a nice concentration profile for each lipase, its minimum and maximum concentration that can produce opaque spots visible to the naked eye should be taken into consideration.
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At this stage the opaque spot gradually disappeared and revealed the transparent part of the glass petri dish beneath it.
Therefore, a concentration profile for the opaque spot intensities could be constructed to further investigate the trend for the hydrolysis spots.
There was also clear observation using naked eyes whereby opaque spot was only formed on the film when there was lipase activity.
The brightness and contrast of image was adjusted and the opaque spot formed on the P 3HB-co-4HB) film was croP 3HB-co-4HBigned and further analyzed using ImageJ, a freeware downloaded from internet.
An early caries lesion on the surface of enamel is observed clinically as a white opaque spot, and hence referred to as a white spot lesion (WSL) [ 20].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com