Sentence examples for opaque interface from inspiring English sources

The cell suspension was centrifuged at 400×g for 30 min at 4°C, and then the opaque interface containing lymphocytes were transferred into a conical centrifuge tube.

In the next stage, these cells were put into falcon and centrifuged at 200×g for 15 min at 4 °C, and then the opaque interface containing lymphocytes was mixed with RBC lysis buffer to eliminate remaining red blood cells.

After centrifugation the opaque interface containing lymphocytes was collected, washed twice in RPMI 1640 and, after complete supernatant removal, the pellet was re-suspended in PBS supplemented with 0.5% of BSA and 2 mmol/L EDTA.

After centrifugation, the opaque interface containing the mononuclear cells was carefully aspirated, washed with RPMI-1640 medium and resuspended in PBS containing 0.2% BSA.

The upper layer was discarded and the opaque interface containing mononuclear cells (buffy coat) was aspirated with a Pasteur pipette and transferred to a new 15 mL centrifuge tube.

Diluted blood with Hank's Buffered Salt Solution (HBSS) was carefully layered onto Histopaque 1077 and centrifuged at 1000 rpm for 10 min. After centrifugation the opaque interface containing mononuclear cells were collected and washed with phosphate buffered saline (PBS).

Even beyond these minor technical difficulties, there are problems: an opaque user interface, poorly explained mechanics, and tiresome battle sequences with no discernable connection to the Pokémon games millions of fans have come to know and love.

It's fun for them to learn about bells and whistles, or to master an opaque user interface (in their saddest delusion, they hope that others will find their advanced tool-use abilities sexy).

After centrifugation the supernatant was discarded and the cell-containing opaque band at the interface between plasma and Histopaque®-1077 Histopaque®-1077refully.

As expected, the aqueous solution of non-cross-linked PTX-CNM3 and PTX-CNM4 solutions became opaque from the aqueous oil interface.

Furthermore, these results indicate that the overall tertiary and/or quaternary structure of CEACAM1 on the cell surface is critical to provide a binding interface for non-opaque meningococci.