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That is, NST should begin first before any of its operations (OP = {OP i | i = 1…n} ∈ NST) can be executed.
A NST can be formally defined as a tuple, NST = (OP, PaO), where OP is a set of operations, OP = {OP i | i = 1…n}, and PaO is a partial ordering of the operations which determines their order of execution.
Consistently, the BrdU labelling index also decreased in ApcΔ 716 op/ op mouse tumours.
To evaluate whether the impaired ductal morphogenesis that occurs in Csf1 op / op mice reflects a perturbation in MaSC activity, freshly sorted CD29hiCD24+ mammary epithelial cells were isolated from BALB/c Csf1 op / op and wild-type glands.
ApcΔ 716 mice were crossed with op/ op mice (The Jackson Laboratory) to obtain ApcΔ 716 op/ op compound mice (n=3) and littermate control ApcΔ 716 mice (n=3).
Both the Csf1 op / op mice and clodronate liposome-mediated ablation models entailed transplantation studies using the MaSC-enriched population.
Interestingly, even in a macrophage-replete system, the function of MaSCs from Csf1 op / op glands was compromised.
Older mutant recipient mice were used to compensate for the marked developmental delay that occurs in Csf1 op / op mice.
Csf1 op / op mice were a kind gift from Dr Robin Anderson Peter MacCallum Cancer Centree, Melbourne, Australia) and were on a pure BALB/c background.
The mammary glands of Csf1 op / op mice display lower numbers of terminal end buds as well as reduced ductal branching and elongation [ 4].
In reciprocal experiments, the MaSC-enriched subpopulation from Csf1 op / op glands had reduced regenerative capacity in a wild-type environment.
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