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Various amounts of each isoform cDNA were injected into oocytes together with GABAB2, GIRK1, and GIRK2.
Nevertheless, we were able to reproducibly obtain live pups when performing IVF with Sirt1−/− sperm or oocytes together with gametes from B6D2F1 mice.
Since the DNA used in this experiment was derived only from oocytes, together with any contaminating follicle cells, the results suggest an association between a demethylated promoter state and activity.
In our study, we investigate the action of 4 insect repellents on the activities of two Ae. aegypti ORs, AaOR2 and AaOR8, respectively, expressed in Xenopus oocytes together with AaOR7.
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The CCs are in physical contact with the oocyte; together they form the cumulus-oocyte complex (COC) and undergo a cross-talk [ 11].
In hierarchical clustering (heatmaps), sixteen oocytes group together, while three oocytes (two immature MI oocytes and one in vitro matured(IVM) oocyte) cluster with seven primitive oocyte-like cells developed in vitro).
Rather than each oocyte developing together with its own complement of 15 nurse cells as in Drosophila, all oocytes in Oncopeltus ovarioles share a common pool of syncytial nurse cells located at the anterior of each ovariole in a region termed the "tropharium" (Fig. 1A1).
(A and B ) Whole cell currents were measured in Xenopus oocytes expressing SLAC1 together with CPK5 and, as a control, CPK6.
To examine whether centrioles overreplicated during oogenesis, we counted centriole numbers in the nurse cells (because the oocyte centrioles clustered together and were difficult to count).
As meiosis completes, six chromosomes remain and form the oocyte pronucleus, which together with the sperm-derived pronucleus contains all the genetic information necessary for the development of a new individual.
A hierarchical clustering confirmed these findings, showing that MII oocytes and hESC clustered together, sharing a signature of overexpressed genes, demonstrating a close gene expression.
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