Sentence examples for onto a gradient from inspiring English sources

The cell suspension was overlaid (1 mL per tube) onto a gradient consisting of 6 mL 30% 'heavy' Percoll™ and 8 mL 10% 'light' Percoll™.

About 30 μg of cell lysate, total protein was loaded onto a gradient 8 15% SDS-polyacrylamide gel.

Tissue was dissociated and cells were loaded onto a gradient made with 30 % and 60 isotonic Percoll.

The gradients were allowed to stand for 3 to 5 hours at 4°C before layering a 0.2-ml sample onto a gradient.

The sample lysates (above) were loaded directly onto the top of a gradient and centrifuged at 200,000 g for 2 hours at 4°C [53].

The magenta trace was obtained after mapping the behavior onto the dynamical reconstruction of the gradient shown in panel B. In contrast, the blue trace was obtained after mapping the behavior onto a static gradient computed 60 s after the onset of the odor diffusion.

Fakhraai, Z., Still, T., Fytas, G. & Ediger, M. D. Structural variations of an organic glassformer vapor-deposited onto a temperature gradient stage.

The P100 was resuspended in 1 ml of 0.211 M sucrose/3 mM imidazole (pH 7.4) buffer, loaded onto a sucrose gradient (0.211 2.255 M sucrose in 3 mM imidazole, pH 7.4), and subject to cfg at 100k × g for 18 h using a swinging bucket rotor (Beckman SW32.1).

The dissociated material was centrifuged, washed, and layered onto a Percoll gradient.

Cleared total cell lysate was denatured by boiling and loaded onto a 4 20% gradient SDS PAGE.

Cell suspensions were again gently passed through a 15 µm sterile steel mesh, and loaded onto a discontinuous gradient consisting of 5 ml of 45% Percoll (Pharmacia) overlaid with 5 ml of 31% Percoll.