Spawning took place in the early morning period after the onset of light.
Furthermore, PhliC bioluminescence induction at the onset of light increased at higher light intensities.
We also found that expression of OsYSL15 was significantly induced in response to Fe deficiency prior to the onset of light and 3 5 h after the onset of light (Fig. 2g, Additional file 1: Table S1A).
Root tissue and root secretions were collected for gene expression and metabolite analyses during the time periods 6 00 8 00 (2–0 h prior to the onset of light), 11 00 13:00 (3–5 h after the onset of light), 18 00 20 00 (10 12 h after the onset of light) and 22 00 24 00 (2–4 h after the change to dark), after one week of Fe deficiency treatment.
Accordingly, Fe deficiency promoted increased secretion of DMA following a diurnal pattern with a peak occurring 3 5 h after the onset of light.
Furthermore, triple quadrupole mass spectrometry identified 3 5 h after the onset of light as peak time of DMA secretion from Fe-deficient rice roots.
Transcripts from Prbp1, Pslr0006, and PsigA increased significantly following the onset of light, whereas transcripts from PhliC exhibited no oscillations in 12 12 LD cycles.
These diurnal patterns are consistent with previous investigations (Nozoye et al. 2004, 2011), and may be summarised as a slight induction of expression prior to the onset of light, a peak of induction 3 5 h after the onset of light, and a return to expression levels similar to that of Fe-sufficient roots prior to dark.
Metabolite profiling identified accumulation of amines associated with metal chelation, metal translocation and plant oxidative stress responses occurring with peak induction 10 12 h after the onset of light.
Thus, annealing at high temperatures clearly induces a not-negligible fall in the light absorption efficiency of Ge QDs, while QD structural phase does not affect the onset of light absorption.
Whilst increased expression of OsYSL15 after the onset of light has been reported previously (Inoue et al. 2009), the diurnal pattern of OsYSL15 that we observed is not consistent with the daily fluctuations of OsYSL15 described by Inoue et al. (2009).
