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The proteins, incorporated into apical and basolateral early endosomes, meet in common endosomes, a process that can be observed within 15 min after the onset of internalization in MDCK cells [4].
The delay between EGF stimulation and onset of internalization of p-EGFR into early endosomes is well documented (Burke et al., 2001; Wiley, 2003).
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As in previous studies of dynamin1 and clathrin in other cell lines, the dynamin fluorescence increased by 65% over a second, coincident with the onset of clathrin internalization (Fig. 3).
We calculated the rate of internalization at the onset as well as after 10 min of incubation at 37°C leading us to similar results obtained by others using radioactive experiments [ 41- 43].
This in turn might reduce the rate of internalization of active neurotoxin and delay the onset of action.
Hence, positively charged NPs should show higher level of internalization.
Degree of internalization of energy-related external costs 38.
The degree of internalization should amount to 100%.
The results of internalization assay was confirmed by MTT assay.
The mechanism of internalization of non-opsonized particles is unknown.
The rate of internalization and/or the rapidity of inactivation of Rho-family proteins in different hosts may partially account for the different rates in the onset of gene expression.
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