Expression of EBF1 and EBF101 was similar to that of EIL1 and EIL3, and did not change significantly over the course of fruit development or at the onset of fruit ripening at 132 days after full bloom (DAFB), though a slight increase in expression was observed at 146 DAFB (Fig. 3).
The duration (in days) of each phenological stage was recorded for both cultivars through visual observations at the following times: the onset of bud sprouting, the onset of flowering, the onset of fruit maturation, the beginning of fruit harvest, the end of flowering, and the end of fruit harvest.
In order to gain molecular insight into early-stage fruit development and to create groundwork for molecular characterization of fruit development, it is desirable to profile the transcriptome of developing fruits of A. squamosa.
In grapevine and tomato, which are classified as non-climacteric and climacteric species respectively, the accumulation of hydrogen peroxide (H2O2) and extensive modulation of reactive oxygen species (ROS) scavenging enzymes at the onset of ripening has been reported, suggesting that ROS could participate to the regulatory network of fruit development.
However, unseeded parthenocarpic fruits show less growth and lack the peak of auxin that occurs before the onset of ripening seen in the normal seeded fruits suggesting that, at least during the later stages of fruit development, the embryo-supplied auxin is necessary for continued fruit growth [ 14, 15].
The duration of fruit development was similar for both cultivars.
fruit (different stages), indicating the possibility of regulation of carotenoid biosynthesis at various stages of fruit development.
This isolation was maintained until the beginning of fruit development.
All four genes have increased expression level during flower and fruit development, particularly a remarkably high expression level of atTOC159 in later stage of fruit development.
