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Thus, while RNA integrity is a poor predictor of performance for RNA from relatively fresh FFPE samples, it provides valuable information about RNA damage and tissue autolysis that occurred prior to embedding, i.e. in the time between resection and the onset of fixation.
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To study LFP activities in response to eye movements, we calculated LFP averages either triggered on the onset of fixations or on the onset of saccades (Fig. 2 C, D ).
Rajkai et al. (2008) reported that the oscillations of neuronal excitability in monkey V1 were phase locked to the onset of fixations during voluntary eye movements performed in the dark, so that the excitability becomes maximal around the time when visual signals should have arrived if the animals were in a lighted environment.
We analyzed neuronal data in three task epochs after onsets of fixation spot, cue, and juice.
The eye positions at the onsets of fixation periods are marked with red dots in Figure 1 A. In the following, we refer to each successive pair of saccade and fixation periods as a saccade fixation (S F) trial.
The fact that the amplitude of the saccade-onset triggered LFP isaccade-onset triggeredion-onset–triggered LFP (fisst peaks, respectivelargerdicathanthet the Lfixation-onset triggeredtightly coupled to the onsets ofixation-onset triggerednsets of fixations.
To examine the changes of the spiking and LFP activities in relation to the eye movements, we computed separately for each of the animals the averages of firing rates and LFPs triggered on the onsets of saccades or onsets of fixations during free viewing of natural images (Fig. 2 A – D ).
In Figure 1 B, we show 10 spike trains and one LFP trace recorded concurrently with the eye movements shown in Figure 1 A. The spectrogram of the LFP trace (Fig. 1 C ) indicates short-lasting power increases in the beta band (10 25 Hz) shortly after onsets of fixations.
The sen1 (symutantstares arrestedsted just before the onset of nitrogen fixation, they form white nodules with no measurable nitrogen fixation activity and they are nitrogen-starved [57].
Although Allopenna et al. reported their data in fixational terms, they in fact calculated the onset of a fixation not in terms of when the eyes first landed on the target, but in terms of when the eyes initiated the saccade which culminated in landing on that target (Magnuson, personal communication).
Trials began with onset of a fixation cross at screen centre.
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