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Six animals per cage weighing 30 40 g each were housed in groups of 10 in our institutional animal care facility and allowed to adapt for seven days prior to the onset of experiments.
HR at rest (HRREST) was individually evaluated before the onset of experiments.
Mice were 9 10 weeks old at the onset of experiments.
Oligodendrocyte precursor cells (OPCs) were prepared by sequential immunopanning and kept under undifferentiating conditions as described earlier [ 45] until the onset of experiments.
The vessels were allowed to equilibrate for 60 minutes before the onset of experiments, with Krebs solution changed every 15 minutes.
To prevent constitutive Ca2+ influx through V102C-Orai1 associatedated mutants from stimulating gene expression during the culture period after transfection, we used the La3+ method (Park et al., 2009), where the CRAC channel blocker La3+ was added to the culture medium and then maintained until shortly before the onset of experiments.
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They have analyzed the soil samples for important soil property parameters at onset of experiment and again after 21 years.
Plants were grown for 10 weeks after onset of experiment and analyzed for mineral composition including B and antioxidation activity.
At the onset of experiment 1, the animals were first-calf heifers pregnant to an Ab bull and in experiment 2 second-calf cows pregnant to a Hereford bull.
Wild type C57BL/6 J (B6) mice were purchased from Jackson Lab (Bar Harbor, ME) and they were 8 12 weeks old at the onset of experiment.
Cell proliferation rate was calculated as follows: [(Aexp – Ablank) – (A0 – Ablank)] /(A0 – Ablank) × 100%, where A0 referred to the absorbance value at the onset of experiment and Aexp referred to the absorbance values of the experimental cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com