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Preceding each run, 4 dummy volumes were acquired, and 1 randomly selected additional melodic block was included to ensure a stable brain state after the onset of each run.
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Four dummy scans were collected prior to the onset of each functional scan run to eliminate the transient effects of magnetic saturation.
Functional data acquisition and the behavioural task began 9 s (three TRs) after the onset of the run.
Because the activity profiles for free running data do not represent the time from the onset of free run during the day of transition and 0 00 am (DAMSystem time) on the first full day of free run, a correction had to be used to calculate free running phases relative to the previous environmental cycle.
The total volume of lava to be erupted is specified at the onset of a model run.
Stimulus presentation was implemented with DMDX software (http://www.u.arizona.edu/∼kforster/dmdx/dmdx.htm), and stimulus presentation was synchronized with the onset of the functional run to ensure accuracy of event timing.
Design expert software produced 25 runs; the composition of each run is given in Table 1.
Nocturnal running reappeared on the second night, but the onset was delayed by 125±91 min relative to the onset of nocturnal running during ad libitum food access (Fig. 3).
Onset of wheel running activity was determined over these 3 days and decapitation and subsequent dissection of the brain was performed at the end of the resting period of the animal (CT 12).
Though previous studies have reported a stimulating effect of running on proliferation under comparable conditions [34], our data are in line with a recent study reporting that proliferation is only increased at the onset of wheel running and returns to baseline levels after 21 days of running [35].
Following preliminary experiments, sound was terminated within 5 s of the onset of wild running behaviour.
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CEO of Professional Science Editing for Scientists @ prosciediting.com